Fig. 3

Zafirlukast effectively mitigates inflammation induced by LPS in epithelial cells in vitro. A A549 cells were subjected to LPS or Zafirlukast (2.5 μM, 5 μM, 10 μM, 20 μM) lasted for 24 h, and the level of IL-1β, IL-6 and TNF-α in each group’s cell supernatant was quantified using ELISA. B A549 cells were subjected to LPS or Zafirlukast (2.5 μM, 5 μM, 10 μM, 20 μM) last for 12 h, and the mRNA expression levels of IL-1β, IL-6, TNF-α and MCP-1 were evaluated through RT-qPCR. C MLE-12 cells were exposed to LPS or Zafirlukast (2.5 μM, 5 μM, 10 μM, 20 μM) for 24 h, the IL-1β, IL-6, and TNF-α levels in the cell supernatant of each group were quantified using ELISA. D MLE-12 cells subjected to LPS or Zafirlukast (2.5 μM, 5 μM, 10 μM, 20 μM) for 12 h, then the mRNA expression levels of IL-1β, IL-6, TNF-α and MCP-1 were assessed using RT-q PCR. Data are shown as mean ± SD (n = 3). Statistical significance was denoted as follows: #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 compared with the control group, and *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with model group