Fig. 4

BTZ increases protein levels of RhoA and RhoC in endothelial cells. (A–F) HUVECs were treated with vehicle or 100 nM BTZ for 6 h. Protein levels of RhoA (A), RhoB (B), and RhoC (C) were assessed using western blot analysis with anti-RhoA, anti-RhoB anti-RhoC, and anti-β-actin antibodies. Representative western blot images are shown at the top of the panel. Relative expression levels of RhoA, RhoB, and RhoC were quantified by normalizing their expression levels to those of β-actin. Data are expressed as fold increases compared to the vehicle-treated cells and shown as means ± s.d. (RhoA, n = 4; RhoB, n = 4; RhoC, n = 3). *, p < 0.05