Fig. 2

Knockdown of NAT10 inhibited cell viability and glycolysis and promoted cell apoptosis of A549 and PC9 cell lines. The transfection efficiency of sh-NAT10 in A549 and PC9 cell lines was detected by A, RT-qPCR and B, Western blot; C, CCK-8 assay was performed to assess the cell viability of A549 and PC9 cell lines; D, Colony formation assay was conducted to analyze the cell proliferation in A549 and PC9 cell lines; E, Quantification of colony number of A549 and PC9 cell lines in each group; F, Flow cytometry was performed to detect the apoptosis of A549 and PC9 cell lines; G, The apoptosis rate of A549 and PC9 cell lines; H, The ¹⁸F-FDG uptake in A549 and PC9 cell lines; I, The lactate production of A549 and PC9 cell lines was detected by specific kit; The ECAR in J, A549 and K, PC9 cell lines. The OCR in L, A549 and M, PC9 cell lines. (n = 3). NAT10, N-acetyltransferase 10; RT-qPCR, reverse transcription-polymerase chain reaction; CCK-8, cell counting kit-8; ECAR, extracellular acidification rate; OCR, oxygen consumption rate; ¹⁸F-FDG, ¹⁸F-fluorodeoxyglucose; sh-RNA, short hairpin RNA