Fig. 2
From: β-elemene inhibits tumor-promoting in small cell lung cancer by affecting M2 macrophages and TGF-β
Effects of β-elemene treatment in M2 and NCI-H209 Co-cultured cells, and interference with TGF-β in M2 macrophages on SCLC migration, viability, and cell cycle. (A) Transwell assay for NCI-H209 cell migration. Scale bar = 100 μm. (B) CCK8 assay to detect NCI-H209 cell viability. (C) qPCR was used to verify the interference effect. (D) Flow cytometry analysis of the cell cycle in NCI-H209 cells. 2 N: Proportion of G0/G1 phase cells to the total cell population; S: Proportion of S phase cells to the total cell population. 4 N: Proportion of G2/M phase cells to the total cell population. * p < 0.05, ** p < 0.01, and *** p < 0.001: compared to the NCI-H209 group. # p < 0.05, ## p < 0.01 and ### p < 0.001: compared to the NCI-H209 + M2 group. ^p < 0.05, ^^^ p < 0.001: compared to the NCI-H209 + M2 + β-elemene group. & p < 0.05 and &&& p < 0.001: compared to the NCI-H209 + M2 + sh-NC group