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Table 1 Interference target site sequence

From: β-elemene inhibits tumor-promoting in small cell lung cancer by affecting M2 macrophages and TGF-β

Target site

Sequence

TGF-β-oligo-1

GCAACAATTCCTGGCGATACC

Top Strand-1

5’-GATCCGGCAACAATTCCTGGCGATACCCTCGAGGGTATCGCCAGGAATTGTTGCTTTTTTG-3’

Bottom Strand-1

5’-AATTCAAAAAAGCAACAATTCCTGGCGATACCCTCGAGGGTATCGCCAGGAATTGTTGCCG-3’

TGF-β-oligo-2

GCAGAGTACACACAGCATATA

Top Strand-2

5’-GATCCGGCAGAGTACACACAGCATATACTCGAGTATATGCTGTGTGTACTCTGCTTTTTTG-3’

Bottom Strand-2

5’-AATTCAAAAAAGCAGAGTACACACAGCATATACTCGAGTATATGCTGTGTGTACTCTGCCG-3’

TGF-β-oligo-3

GGACATCAACGGGTTCACTAC

Top Strand-3

5’-GATCCGGGACATCAACGGGTTCACTACCTCGAGGTAGTGAACCCGTTGATGTCCTTTTTTG-3’

Bottom Strand-3

5’-AATTCAAAAAAGGACATCAACGGGTTCACTACCTCGAGGTAGTGAACCCGTTGATGTCCCG-3’

  1. Note: Loop Sequence in shRNA is: CTCGAG
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BMC Pulmonary Medicine

ISSN: 1471-2466

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