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Fig. 6 | BMC Pulmonary Medicine

Fig. 6

From: Development and validation of a Guinea pig model for concurrent allergic rhinitis and asthma using recombinant Der f 2

Fig. 6

Increased MUC5AC expression in airway tissues (nasal and lung) of guinea pigs sensitized and challenged with Der f 2. a - c. Nasal mucosa: Paraffin-embedded nasal mucosa from control (a) and experimental (b) guinea pigs, sacrificed 2 h after the final intranasal instillation of normal saline or Der f 2, were sectioned, incubated with a mouse anti-MUC5AC antibody, and visualized using an ABC kit. Five microscopic fields per section were captured at 200× magnification. Images were analyzed using Image-Pro Plus 12.0 (Media Cybernetics, Rockville, MD, USA), and the integrated optical density (IOD) of the positive regions was calculated to determine the mean optical density (MOD) (c). (**p < 0.01). d-f. Lung tissues: Paraffin-embedded lung tissues from control (d) and experimental (e) guinea pigs, sacrificed 2 h after the final intranasal instillation of normal saline or Der f 2, were stained with an anti-MUC5AC antibody and visualized using an ABC kit. MUC5AC expression is presented as the MOD ± SD (f). (**p < 0.01)

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BMC Pulmonary Medicine

ISSN: 1471-2466

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