Fig. 2

Differential expression analysis of m6A-modified circRNAs in CIH and control groups. A Heatmap of differentially expressed circRNAs modified by m6A, showing hierarchical clustering of samples from CIH and control groups. Red indicates up-regulated circRNAs, and blue indicates down-regulated circRNAs (|FC| ≥ 2, p < 0.05). The heatmap demonstrates clear separation between the CIH and control groups, indicating distinct expression patterns. B Volcano plot showing the distribution of circRNAs based on fold change and statistical significance. Up-regulated circRNAs are shown in red, and down-regulated circRNAs are shown in blue, with gray dots indicating non-significant changes. The plot highlights the significant differences in circRNA expression between the two groups. C Scatter plot of normalized signal intensities of circRNAs in the two groups, highlighting the distribution of differentially expressed m6A-modified circRNAs (|FC| ≥ 2, p < 0.05). The scatter plot illustrates the overall expression trends and the degree of variation between the groups