Fig. 1

Identification of exosomes from Tregs of Sprague Dawley rat spleen. (A) Flow cytometry was used to identify cells. Flow quadrant diagram of Tregs (quadrant 2). (B) Transmission electron microscope was used to observe morphology of exosomes and the representative images of exosomes under transmission electron microscope were shown. Magnification ×60k, scale bar = 100 and 50 nm. (C) Nanoparticle Tracking Analysis was used for particle size distribution of exosomes. There was a total of 21,714,571 events. n = 5. (D) Western blot was used to detect exosome biomarkers and there were representative band images of exosome biomarkers CD63, CD9, and TSG101. n = 3. The “n” means number of experimental replicates