Fig. 5

The effect of normal Treg-exo on CD4 + T-cells from spleen of COPD rats. (A) Flow cytometry assay was used for CD4⁺ T-cell identification, n = 3. (B) Flow cytometry was used for Th17 (CD4⁺ IL-17⁺ cells) identification and CD4⁺ IL-17⁺ cells proportion analysis, n = 3. (D-F) The contents of (D) IL-6, (E) IL-17, and (F) IL-23 were detected using ELISA. n = 6. (G-I) The relative expressions of (G) STAT3, (H) p-STAT3, and (I) IL-17 were measured using Western blot. n = 3. (J) Representative image of protein bands. n = 3. The “n” means number of experimental replicates. Mean ± standard deviation, one-way analysis of variance was used for statistical analysis. ^**p < 0.01 vs. control group; ^#p < 0.05, ^##p < 0.01 vs. model group