Correction: Zafirlukast ameliorates lipopolysaccharide and bleomycin-induced lung inflammation in mice

Correction: BMC Pulm Med 24, 456 (2024)

https://doi.org/10.1186/s12890-024-03273-6

Following publication of the original article [1], the authors identified an error in Fig. 1A (H&E lung) and Fig. 4. The correct figures are given below.

The original article has been corrected.

Fig. 1

Zafirlukast attenuates acute lung injury induced by LPS in mice. A H&E staining of lung tissue segments (Scale: 50 μm) and the BALF cell of mice (Scale: 50 μm). B Total cell counts in BALF of mice. C-E Number of inflammatory cells, lymphocytes, neutrophils, and macrophages. F Protein concentration in BALF of mice. G–J MCP-1, IL–1β, IL-6 and TNF-α mRNA expression in lung tissues of mice. K-M Measure the protein level of inflammatory factors IL–1β, IL-6 and TNF-α in BALF using ELISA. The data which presented in this study are expressed as mean ± SD (n = 3). the statistical analysis revealed significant differences when compared with the control group, denoted as #P < 0.05, ##P < 0.01, ###P < 0.001, *P < 0.05, **P < 0.01, ***P < 0.001 as compared with model group

Fig. 4

Zafirlukast effectively inhibits the LPS-induced inflammation of epithelial cells through TLR4/NF-KB/NLRP3 pathway in vitro. (A-B) A549 cells were subjected to different concentration of LPS or Zafirlukast (2.5µM, 5µM, 10µM, 20µM) for a duration of 24 h, Western blotting was then performed for assessing the protein expression levels of MyD88, TLR4, P65, P-P65 as well as inflammasome pathway-related protein NLRP3, caspase1, Cleaved-caspase1, IL-1β and Cleaved-IL-1β in the cells (A). The quantification of optical density was also determined (B). (C-D) Additionally, the activation of MYD88 (C) and P-P65 (D) by immunofluorescence in A549 cells subjected to LPS or Zafirlukast (2.5µM, 5µM, 10µM, 20µM) for 24 h (Scale: 25 μm). (E-F) MLE-12 cells were subjected to LPS or Zafirlukast (2.5µM, 5µM, 10µM, 20µM) for 24 h, and the Western blotting was used to assess the MyD88, TLR4, P65, P-P65 and the inflammasome pathway related protein NLRP3, caspase1, Cleaved-caspase1, IL-1β and Cleaved-IL-1β protein expression levels in the cells (E). The optical density was quantified and presented (F). (G-H) MLE-12 cells were subjected to LPS or Zafirlukast (2.5µM, 5µM, 10µM, 20µM) for 24 h, and the activation of MYD88 (G) and P-P65 (H) was assessed through immunofluorescence (Scale: 25 μm). Data are shown as mean ± SD (n = 3). Statistical significance was denoted as follows: #P < 0.05, ##P < 0.01, ###P < 0.001 compared with control group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with model group